During this presentation, my intention is to acquaint you with Electron Paramagnetic Resonance Imaging (EPRI), a versatile imaging modality that facilitates the study of free radical molecules or atoms from materials, ranging from in-vitro to in-vivo applications in biomedical research. Despite recent advancements in EPRI techniques, which have enabled the examination of a single free radical, or source, inside the imaging device, the reconstruction of multiple sources, or source separation, remains a complex task. Currently, the state-of-the-art technique relies heavily on time-consuming acquisition and voxel-wise direct inverse methods, which are prone to artifacts and do not utilize the spatial consistency of the source images for reconstruction. To address this issue, we propose a variational formulation of the source separation problem with Total Variation as an a priori constraint that emphasizes the spatial consistency of the source. This approach significantly reduces the number of required acquisitions without compromising the quality of the source separation. An EPRI experimental study has been conducted, and we will present some of the results obtained.